Standard curve Guide, Meaning , Facts, Information and Description
A standard curve is a quantitative research tool that is used to determine the concentration of a substance, particularly proteins and DNA.The asssy is first performed with various known concentrations of the substance being measured, for example a standard curve for protein concentration is often created using know concentrations of bovine serum albumin. The assay may require measurment of absorbance, optical density, luminescence, fluorescence, radioactivity, or something else. The assay for protein is called the Bradford assay, it is a colourmetric assay. The reagent coomassie brilliant blue it turns blue when it binds to arginine and aromatic amino acids present in protein, the intensity of the colour is measured at 595 nm with a spectrophotometer. In the case of the Bradford assay the greater the absorbance, the higher the protein concentration.
This data is used to make the standard curve, concentration on the X axis, and assay measurement on the Y axis. The same assay is then performed with samples of unknown concentration. To analyze the data, locate the measurement on the Y-axis that corresponds to the assay measurement of the unknown substance, follow a line to intersect the standard curve and read down the graph until you intersect the X-axis. The value on the X-axis is the concentration of substance in the unknown sample.
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